Biogeochemical Instrument Laboratory
Biogeochemical Instrument Laboratory

Biogeochemical Instrument Laboratory

 

       El servicio de Análisis de Técnicas Instrumentales Biogeoquímicas del Museo Nacional de Ciencias Naturales – CSIC de Madrid está especializado en determinaciones físicas y químicas en muestras líquidas (aguas naturales) y sólidas (sedimentos y plantas) para el estudio ecológico y biogeoquímico de los ecosistemas acuáticos continentales. Presta su asistencia tanto a grupos de investigación del MNCN y de otros centros del CSIC como a otros organismos o entidades públicas o privadas, que así lo soliciten. El servicio cuenta con un Analizador Elemental Perkin Elmer 2400 Serie II, un Espectrofotómetro Lambda 35 (Perkin Elmer) que opera un amplio rango del espectro UV y visible con doble haz de luz, un analizador de Carbono Orgánico Total marca Shimadzu, modelo TOC-V CSH que lleva acoplado un módulo de detección de Nitrógeno Total para la serie TOC-V, un Analizador de flujo segmentado en continuo SEAL Analytical AutoAnalyzer 3 y un Cromatografo de Gases Agilent Technologias 6890N. Aparte, el servicio cuenta con toda la equipación necesaria para llevar a cabo  los análisis químicos (estufa, mufla, baños de arena, placas calefactoras, pH-metro, centrífugas, etc). El laboratorio está dotado con una balanza analítica (alcance 120 g; d 0,0001) Mettler-Toledo ML104/01 y una micro balanza (alcance 6g; d 0,000001) Mettler-Toledo XP6.

       The laboratory is located in the ICA building (5th floor) and supports research on the biogeochemistry of a variety of systems, but particular attention is focused on aquatic systems.  The laboratory has the potential to perform a wide range of soil, vegetation and water chemistry analyses commonly associated with elemental cycling research.  Equipment includes chemical instrumentation for quantifying different analyte concentrations, drying ovens, incubators, balances, a muffle furnace, filtering apparatus, vacuum hoods, sampling devices, PC's and printers.   

Available analyses in the laboratory:

 

 

1) In water samples:

  • Total organic carbon (TOC) in water samples, including particulate organic carbon (POC), dissolved organic carbon (DOC) and total nitrogen (TN)
  • ​Ammonium, nitrite, nitrate, orthophosphate and total phosphorus (TP),  following APHA (2005)
  • Total solids (110 ºC), total mineral and organic matter (loss of ignition 550 ºC), suspended solids, carbonates-bicarbonates, clorides, sulfates, calcium, magnesium, sodium, potasium, silica, total, temporary and permanent hardness, following APHA (2005)
  • Water colour (ʎ = 440 nm, in mg Pt/l)
  • Chlorophyll-a (after methanol extraction, following Maker et al. (1980)
  • Dissolved oxygen by the Winkler (tritation) method, following APHA (2005)

 

2) In soil/sediment samples:

  • Soil water content (gravimetric method)
  • Elemental analysis of C, N and P (after acid digestion using ammonium persulfate/sulfuric acid)
  • Nutrient extraction (nitrogen with CaCl2 and assimilable phosphorus with the Olsen method) for further analysis following previous description (see 3)
  • Carbonates using the Bernard calcimeter
  • Microbial biomass C, N and P by chloroform fumigation-incubation method (Brookes et al. 1985, Inubushi et al. 1991, Ivanoff et al. 1998, Witt et al. 2000, Truu et al. 2009, Sánchez-Carrillo et al. 2014) and then measured by the Shimadzu TOC-V analyzer (C and N) and by the SEAL autoanalyzer (P, after acid digestion; see above)

 

3) In plant samples:

  • Chlorophyll-(a+b) after methanol extraction and following Porra, Thompson and Kriedemann (1989)
  • Elemental analysis of C, N and P (after acid digestion using ammonium persulfate/sulfuric acid)​
  •  

     To contact with the Lab for any question, support or analysis request, please e-mail to José Luis Ayala (jlayala@mncn.csic.es)

Contact
The website encountered an unexpected error. Please try again later.
Drupal\Core\Database\DatabaseExceptionWrapper: SQLSTATE[HY000]: General error: 1114 The table 'watchdog' is full: INSERT INTO {watchdog} (uid, type, message, variables, severity, link, location, referer, hostname, timestamp) VALUES (:db_insert_placeholder_0, :db_insert_placeholder_1, :db_insert_placeholder_2, :db_insert_placeholder_3, :db_insert_placeholder_4, :db_insert_placeholder_5, :db_insert_placeholder_6, :db_insert_placeholder_7, :db_insert_placeholder_8, :db_insert_placeholder_9); Array ( [:db_insert_placeholder_0] => 0 [:db_insert_placeholder_1] => cron [:db_insert_placeholder_2] => Starting execution of @module_cron(). [:db_insert_placeholder_3] => a:1:{s:7:"@module";s:10:"aggregator";} [:db_insert_placeholder_4] => 5 [:db_insert_placeholder_5] => [:db_insert_placeholder_6] => http://mncn.bmtest.es/en/investigaci%C3%B3n/servicios-cientifico-tecnicos/biogeochemical-instrument-laboratory [:db_insert_placeholder_7] => [:db_insert_placeholder_8] => 127.0.0.1 [:db_insert_placeholder_9] => 1731846091 ) in Drupal\dblog\Logger\DbLog->log() (line 78 of core/modules/dblog/src/Logger/DbLog.php). 
Drupal\dblog\Logger\DbLog->log(5, 'Starting execution of @module_cron().', Array) (Line: 103)
Drupal\dblog\Logger\DbLog->log(5, 'Starting execution of @module_cron().', Array) (Line: 127)
Drupal\Core\Logger\LoggerChannel->log(5, 'Starting execution of @module_cron().', Array) (Line: 99)
Drupal\Core\Logger\LoggerChannel->notice('Starting execution of @module_cron().', Array) (Line: 222)
Drupal\Core\Cron->invokeCronHandlers() (Line: 134)
Drupal\Core\Cron->run() (Line: 75)
Drupal\Core\ProxyClass\Cron->run() (Line: 65)
Drupal\automated_cron\EventSubscriber\AutomatedCron->onTerminate(Object, 'kernel.terminate', Object)
call_user_func(Array, Object, 'kernel.terminate', Object) (Line: 111)
Drupal\Component\EventDispatcher\ContainerAwareEventDispatcher->dispatch('kernel.terminate', Object) (Line: 88)
Symfony\Component\HttpKernel\HttpKernel->terminate(Object, Object) (Line: 32)
Stack\StackedHttpKernel->terminate(Object, Object) (Line: 686)
Drupal\Core\DrupalKernel->terminate(Object, Object) (Line: 22)